High-throughput polyribosome fractionation.
نویسندگان
چکیده
Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose gradients prepared in deep 96-well plates, the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneously in a tabletop centrifuge.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 32 10 شماره
صفحات -
تاریخ انتشار 2004